Non-thermal atmospheric pressure plasma preferentially induces apoptosis in p53-mutated cancer cells by activating ROS stress-response pathways.

Non-thermal atmospheric pressure plasma preferentially induces apoptosis in p53-mutated cancer cells by activating ROS stress-response pathways.

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Non-thermal atmospheric pressure plasma (NTAPP) is an ionized gas at room temperature and has potential as a new apoptosis-promoting cancer therapy that acts by generating reactive oxygen species (ROS).However, it is imperative to determine its selectivity and standardize the components and composition of NTAPP.Here, we designed an NTAPP-generating apparatus combined with a He gas feeding system and demonstrated its high selectivity toward p53-mutated cancer cells.We first determined the proper conditions for NTAPP exposure to selectively induce apoptosis in cancer cells.

The apoptotic effect of Masturbators NTAPP was greater for p53-mutated cancer cells; artificial p53 expression in p53-negative HT29 cells decreased the pro-apoptotic effect of NTAPP.We also examined extra- and intracellular ROS levels in NTAPP-treated cells to deduce the mechanism of NTAPP action.While NTAPP-mediated increases in extracellular nitric oxide (NO) did not affect cell viability, intracellular ROS increased under NTAPP exposure and induced apoptotic cell death.This effect was dose-dependently reduced following treatment with ROS scavengers.

NTAPP induced apoptosis even in doxorubicin-resistant cancer cell lines, demonstrating the feasibility of NTAPP as a potent cancer therapy.Collectively, these results strongly support the potential of NTAPP as Long Waterproof Coats a selective anticancer treatment, especially for p53-mutated cancer cells.